Cotton. Photo courtesy of Chinese Academy of Agricultural Sciences
Recently, the cotton molecular genetic improvement innovation team of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences established a high-efficiency genetic transformation system (SAMT) for cotton without genotype restriction across interspecies and intraspecific species, which solved the bottleneck problem that it is difficult to use the main cotton cultivars as the recipient for genetic transformation, and provided technical support for accelerating the process of cotton genetic engineering breeding. The results were published in the Journal of Integrative Plant Biology.
Genotype restriction and long transformation cycle are the two major technical barriers of cotton genetic transformation, which hinder the verification of cotton gene function and the creation of excellent transgenic materials, and often cannot directly improve the target traits of the current main cotton cultivars, and it is difficult to achieve gene aggregation and variety improvement based on the main cultivar.
In this study, cotton seed apical meristem cells were used as explants, combined with agrobacterium and ultrasonic treatment, the exogenous vector was integrated into stem cells, and then induced stem cells to produce adventitious buds, and spectinomycin was used to inhibit the growth of main buds and induce the production of axillary buds, effectively reducing the probability of chimera.
The SAMT transformation system established by this study successfully broke the genotype limitation of interspecific and intraspecific genetic transformation of cotton, and many cotton varieties such as land cotton, island cotton and Asian cotton were successfully transformed, and the overexpressed transgenic materials and gene-editing mutant materials obtained could be stably inherited.
The transformation cycle of SAMT system was 2~3 months, and no sterility of transgenic regenerated seedlings occurred, and no malformed seedlings appeared. The establishment of this system will help accelerate the verification of cotton functional genes and the creation of excellent germplasm resources, and provide scientific and technological support for cotton biological breeding.
The research was supported by the National Natural Science Foundation of China Innovation Research Group, the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences, and the Yazhou Bay Seed Laboratory Unveiling Project. (Source: China Science News, Li Chen, Liang Bing)
Related paper information:https://doi.org/10.1111/jipb.13427