Caption: The culture process of human intestinal organoids. Photo by Junichi Takahashi
In science fiction movies, you can always see scenes of scientists cultivating human organs in laboratories. Currently, Japanese scientists have developed a new method to make efficient cultivation of human intestinal organoids a reality.
In a study published today in Cell Reporting Methods, researchers at Tokyo Medical and Dental University found that using some specialized experimental techniques, it is possible to produce intestinal-like tissue with predictable size and composition.
Organoids are collections of organ-specific cells. It is a model based on a 3D in vitro cell culture system that is highly similar to the source tissue or organ in vivo. At the same time, it has similar physiological responses to differentiated tissues and organs in the body, and has a high degree of similarity with source tissues. Related research has broad prospects in the field of regenerative medicine.
Junichi Takahashi, first author of the study, said: “Traditional methods can grow human intestinal organoids from induced pluripotent stem cells. “However, the execution of this method is quite challenging: it produces spheres of various sizes and is limited by growth conditions; Over time, spheres can become deformed and unhealthy. “
To develop a more stable and efficient method for growing human gut organoids, the researchers used cell culture plates made of ultra-low adhesion polymers to facilitate cell separation and growth in suspension. They tested the effects of growing spheroids in bioreactors, a specialized incubator that keeps growth media flowing and improves cell health.
Tomohiro Mizutani, corresponding author of the study, said: “Using our technique, predictable, consistent-sized spheroids can be grown and can also be altered by regulating the number of cells. “In addition, transferring spheroids into bioreactors allows them to grow larger and become healthy human gut organoids.”
When these organisms are transplanted into mice, they continue to grow and differentiate and form complex structures that reflect a mature gut. “Our study shows that by inducing spheroids in suspension and maturing them in bioreactors, intestinal tissue can be generated from human intestinal organoids derived from induced pluripotent stem cells,” Takahashi said. ”
Given that traditional techniques have been able to generate more complex intestinal tissue, this new method may be used to grow more complex organoids, such as intestinal tissue containing blood vessels or nerves. These laboratory-grown tissues have broad application prospects in future regenerative medicine. (Source: Meng Lingxiao, China Science News)
Related paper information:https://doi.org/10.1016/j.crmeth.2022.100337