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From China:Shanghai Institute of Microsystems has made progress in in situ fluorescence detection of exosomes miRNA

Exosomes are considered as potential tumor diagnostic markers due to their wide sources, stable structure, rich contents and important role in information and substance transfer between cells. Therefore, emerging exosomes and exosome content detection technologies are emerging in an endlessly. In recent years, a variety of based on the analysis of the fluorescent probe techniques have been developed and successfully applied to secrete body outside condition in situ analysis of micrornas, but these methods secrete the nanoscale body work out unique constraints is still difficult to solve, in limited space, high frequency Brownian motion and the collision cause undesired fluorescence quenching and fluorescence resonance energy transfer, lead to signal distortion. Therefore, it is urgent to develop a new fluorescent probe to overcome the above difficulties.

Recently, mi Xianqiang’s research team at the Shanghai Institute of Microsystems and Information Technology, Chinese Academy of Sciences, in collaboration with Tongji University and Shanghai University, A Cubic DNA nanocage-based three-dimensional Molecular beacon (ncMB) with stable structure and high programmable height was constructed. The steric hindrance and electrostatic repulsion generated by the unique three-dimensional structure of ncMB constitute a barrier between fluorescent probes, which can eliminate single-species Molecular beacons. MB) undesired fluorescence quenching between fluorescence molecules and quenched molecules during detection and undesired fluorescence resonance energy transfer between donor fluorescence molecules and recipient fluorescence molecules based on multiple MB detection, thus solving the signal distortion problem. Due to its excellent anti-fluorescence quenching and anti-fluorescence resonance energy transfer properties, the fluorescence signal obtained by ncMB in exosome detection can be improved by an order of magnitude compared with traditional MB. In addition, ncMB has strong programmability and anti-interference capability. Therefore, ncMB is expected to be a powerful and versatile tool for accurate quantitative analysis of markers in confined Spaces represented by exosomes.

The Cubic DNA Nanocage-based three-dimensional Molecular Beacon was used for accurate detection of Exosomal miRNAs in confined devices Spaces was published in Biosensors and Bioelectronics.

Paper Link & NBSP;

Shanghai Institute of Microsystems has made progress in in situ fluorescence detection of exosomes miRNA

Exosomes are considered as potential tumor diagnostic markers due to their wide sources, stable structure, rich contents and important role in information and substance transfer between cells. Therefore, emerging exosomes and exosome content detection technologies are emerging in an endlessly. In recent years, a variety of based on the analysis of the fluorescent probe techniques have been developed and successfully applied to secrete body outside condition in situ analysis of micrornas, but these methods secrete the nanoscale body work out unique constraints is still difficult to solve, in limited space, high frequency Brownian motion and the collision cause undesired fluorescence quenching and fluorescence resonance energy transfer, lead to signal distortion. Therefore, it is urgent to develop a new fluorescent probe to overcome the above difficulties.

Recently, mi Xianqiang’s research team at the Shanghai Institute of Microsystems and Information Technology, Chinese Academy of Sciences, in collaboration with Tongji University and Shanghai University, A Cubic DNA nanocage-based three-dimensional Molecular beacon (ncMB) with stable structure and high programmable height was constructed. The steric hindrance and electrostatic repulsion generated by the unique three-dimensional structure of ncMB constitute a barrier between fluorescent probes, which can eliminate single-species Molecular beacons. MB) undesired fluorescence quenching between fluorescence molecules and quenched molecules during detection and undesired fluorescence resonance energy transfer between donor fluorescence molecules and recipient fluorescence molecules based on multiple MB detection, thus solving the signal distortion problem. Due to its excellent anti-fluorescence quenching and anti-fluorescence resonance energy transfer properties, the fluorescence signal obtained by ncMB in exosome detection can be improved by an order of magnitude compared with traditional MB. In addition, ncMB has strong programmability and anti-interference capability. Therefore, ncMB is expected to be a powerful and versatile tool for accurate quantitative analysis of markers in confined Spaces represented by exosomes.

The Cubic DNA Nanocage-based three-dimensional Molecular Beacon was used for accurate detection of Exosomal miRNAs in confined devices Spaces was published in Biosensors and Bioelectronics.

Paper Link & NBSP;

Shanghai Institute of Microsystems has made progress in in situ fluorescence detection of exosomes miRNA

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