Important progress has been made in the field of biosensing based on light manipulation

Cover of the current issue. Photo courtesy of the research team

Recently, Professor Zheng Xianchuang and Associate Professor Liu Xiaoshuai of the Institute of Nano Photonics of Jinan University have made important progress in the field of biosensing based on light manipulation. The research was published in Advanced Materials and featured in the cover paper. Zhang Tiange, a doctoral student at the Institute of Nanophotonics of Jinan University, is the first author of the paper.

In this study, a light-controlled virtual microsensor (OCViM) that can be used for scanning detection and quantitative analysis of multiple biomarkers in living blood vessels is constructed by combining optical manipulation technology and molecular imaging methods, using a focused Gaussian beam as a virtual controller and a synthetic nanofluorescent probe as a micro-nano sensing probe.

Real-time detection of biomarkers in vivo is essential for characterizing physiological mechanisms and pathological processes in vivo organisms. At present, mainstream strategies such as needle-tip microelectrodes and molecular imaging methods require inserting tens to hundreds of microns into the living body or passively relying on the random distribution of imaging probes, which are highly invasive, lack of spatial resolution, and lack of active manipulation, respectively.

In view of the above challenges, the researchers combined the scanning optical tweezer technology of time-multiplexing and the upconversion fluorescence detection technology of stimulus response, and proposed and developed a light-controlled virtual microsensor that can be used for real-time detection of multiple biomarkers in living blood vessels. Using the constructed virtual microsensor, they successfully realized the real-time detection of the expression level and heterogeneity distribution of biomarkers on the zebrafish thrombosis model, and comprehensively evaluated the therapeutic effect of antithrombotic drugs, which provided a powerful tool for studying the mechanism of thrombosis and the efficacy evaluation of antithrombotic drugs.

This virtual microsensor based on optical manipulation and fluorescence excitation is like a “nano lantern” that can illuminate lesions in blood vessels, with the help of optical manipulation of this invisible hand, it can realize non-invasive, high temporal precision, programmable and multi-functional quantitative detection of specific biomarkers at different lesions, which is expected to provide a new method for real-time detection of biomarkers in vivo, and has potential application prospects in the fields of mechanism research and drug development of multiple diseases. (Source: China Science Daily, Zhu Hanbin, Su Qianyi)

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