LIFE SCIENCE

It was found that strain XN08 had biological control potential for wheat-veined blight


GFP-labeled bidirectional Burkholderia colonization characteristics in wheat seedlings. Morphology of GFP-labeled strain (A) under fluorescence confocal microscopy, comparison of strain antagonism and antagonism activity with wild strains (B-C), morphological characteristics of wheat seedlings under different growth cycles (D), colonization confocal microscopy observation of marker strains in the root system of wheat at 2 days after inoculation (E: brightfield, F: darkfield), and 7 days (G: brightfield, H: darkfield).

Pathogenic fungal antagonistic and plant-promoting properties of Bi-Directional Burkholderia XN08 in pot experiments. Comparison of wheat morphological characteristics (A), plant plant height (B), plant fresh weight (C), and plant-induced resistance enzymes PPD(D), POD(E), PAL(F) under different treatment conditions. (Images courtesy of the author)

Wheat blight caused by Rhizoctonia cerealis is the most common necrotizing wheat soil facsimile disease, which generally causes a loss of wheat yield by 10% to 40%. The application of plant probiotic bacteria to control wheat blight as a green sustainable agricultural biotechnology has attracted a lot of attention. However, there are still limitations in the practical application of grass silkensis, one of the main reasons is that some probiotic agents are difficult to effectively colonize in plants after indirect planting in the field, so that the biocontrol and pro-growth effect cannot be guaranteed.

The Xue Wenjiao research group of Shaanxi Institute of Microbiology recently published a research paper online in Frontiers in microbiology, which systematically evaluated the biological control potential of the endophytic burkholderia ambifaria XN08 on wheat blight, laying the foundation for the research and development of wheat blight biocontrol agents.

The research group obtained burkholderia ambifaria XN08 by screening the antibacterial strain of Corticosteria coleus, and for the first time found that the strain could be effectively colonized in wheat tissue by constructing an eGFP (Enhanced Green Fluorescent Protein) labeled strain with stable genetic characteristics. In addition, they further showed through the amplification of antibiotic synthesis genes and the analysis of the metabolite UPLC-MS that the strain XN08 can produce the antibacterial substance nipyrrolidin and found that the strain can produce plant bio-promoting substances including IAA, protease, ferrophilin and other plants through in vitro experiments, and then found that the effective colonization of the strain in plant tissues can induce plants to produce resistant proteins, and the effect on the prevention and control of wheat blight is obvious.

“The overall findings show that plant-promoting strain XN08 has good biological control potential for wheat-veined blight.” Xue Wenjiao summarized the explanation.

The paper was completed by Shaanxi Institute of Microbiology, with An Chao as the first author and Xue Wenjiao as the corresponding author. This work has been funded by the National Natural Science Foundation of China and the Outstanding Youth Scientific Research Project and Youth Joint Innovation Project of the Shaanxi Academy of Sciences. (Source: China Science Daily, Zhang Xingyong, Yan Tao)

Related paper information:https://doi.org/10.3389/fmicb.2022.906724



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