INFORMATION TECHNOLOGY

Kiwifruit high-density SNP genotyping chip was successfully developed


New germplasm of soft date kiwifruit. Photo courtesy of Zheng Guo, Chinese Academy of Agricultural Sciences

Soft date kiwi fruit of different genotypes. Photo courtesy of Zheng Guo, Chinese Academy of Agricultural Sciences

Fruits of different kiwifruit germplasm resources. Photo courtesy of Zheng Guo, Chinese Academy of Agricultural Sciences

Recently, the kiwifruit resource and breeding team of the Zhengzhou Institute of Pomology of the Chinese Academy of Agricultural Sciences published a research paper in the Plant Biotechnology Journal. Through the development of high-density SNP genotyping chips of kiwifruit, the evaluation of kiwifruit germplasm resources and the genetic characteristics of tetraploid soft jujube kiwifruit were carried out, which provided an important reference for subsequent molecular marker-assisted breeding and genetic mechanism of important traits of kiwifruit.

Kiwi fruit belongs to perennial deciduous fruit trees, with dioecious, highly heterozygous, self-inbred incompatibility, long breeding cycle and other characteristics, domestic and foreign breeders still rely on traditional breeding methods, such as early use of wild direct selection or live selection and other methods, these methods are more blind. Opening up new ways of genetic improvement and broadening the genetic base of cultivated kiwifruit will help reduce breeding costs and diversify varieties, and promote the quality and efficiency improvement and sustainable and healthy development of the kiwifruit industry.

Based on the resequencing data of kiwifruit germplasm resources, a high-density SNP genotyping chip was developed and the kiwifruit germplasm resources were identified. At the same time, the F1 population of tetraploid soft jujube kiwifruit (2n=4x=116) was genotyped to construct a high-density integrated genetic map.

The results showed that the genomes of tetraploid soft jujube kiwi and diploid Chinese kiwifruit maintained a high degree of collinearity, localized to male-specific regions, and further obtained molecular markers closely linked to it. By analyzing the isolation pattern and chromosome pairing of different dose markers in offspring, it was found that there was a preference for chromosome pairing in the process of meiosis in Homotetraploid soft date kiwifruit, which provided a strong basis for further research on the special genetic phenomenon of polyploidy.

Fang Jinbao, researcher of Zhengzhou Institute of Pomology, Chinese Academy of Agricultural Sciences, Chris Maliepard, Department of Plant Breeding of Wageningen University in the Netherlands, are the corresponding authors of the paper, and Wang Ran, assistant researcher of Zhengzhou Institute of Pomology, Chinese Academy of Agricultural Sciences, is the first author. The research has been supported by the National Key Research and Development Project, the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences, and the National Natural Science Foundation of China. (Source: Li Chen, China Science News)

Related paper information:https://doi.org/10.1111/pbi.13958



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