Researchers have developed a real-time monitor of clinical hypersensitive exhaled ammonia

Recently, Li Haiyang, a researcher at the Dalian Institute of Chemical Physics, Chinese Academy of Sciences, and Leng Song’s team, a professor at the Second Affiliated Hospital of Dalian Medical University, developed a real-time monitor and new method for point-of-care diagnosis based on the high-resolution ion mobility spectroscopy technology independently developed by the Dalian Institute of Chemical Sciences, realizing the high-sensitivity and high-specificity real-time monitoring of exhaled ammonia during periodic breathing.

This method can effectively reduce the interference of high humidity, complex background and high adsorption residue of small molecule ammonia on the detection results in exhaled breath, and provide a non-invasive, real-time and accurate new instrument and method for the detection of ammonia, an important biological metabolic marker in the human body. The results were published in the Journal of Pharmacological Analysis.

Exhaled ammonia real-time monitor (photo provided by the research team)

Exhaled ammonia is closely related to various important physiological processes in the body, such as amino acid synthesis-metabolism, urea-nitrogen homeostasis, and blood acid-base balance buffering. Moreover, the concentration of ammonia in the exhaled breath provides an important reference for the diagnosis of liver and kidney function, Reye’s syndrome, urea cycle disorders, organic acidosis, and Helicobacter pylori infection. Therefore, rapid, non-invasive, and accurate quantitative monitoring of exhaled ammonia is of great clinical significance.

In this work, the team modulated the ionic-molecular reaction by adding the modifier acetone to the bleaching gas, significantly improving the peak-peak resolution of ammonia and reagent molecules, and achieving high-specificity detection of trace ammonia in thousands of exhaled components. At the same time, the team developed online dilution and purge sampling technology, which solved the problem of adsorption residue of ammonia molecules and realized the highly sensitive detection of exhaled ammonia at 100% RH. Moreover, accurate quantitative detection of exhaled ammonia can be achieved in a wide concentration range, and the single analysis time is only 40ms.

Compared with the current blood ammonia concentration detection method, the exhaled ammonia ion mobility spectrometer has the advantages of non-invasive detection, strong real-time performance, good selectivity and high sensitivity, which is especially suitable for real-time monitoring of dialysis efficacy and early identification of hepatic encephalopathy, showing the important application value of point-of-care diagnosis.

At present, the instrument has been carried out health detection and evaluation in the Health Management Medical Center of the Second Affiliated Hospital of Dalian Medical University. (Source: Sun Danning, China Science News)

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