Researchers have developed fully automatic low-temperature digital microfluidic equipment

Recently, the latest research results of Yang Hui, a researcher at the Institute of Medical Engineering of Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, and Chen Hong’s team at the Sabendong Institute of Micro and Nano of Xiamen University, were published in Lab-on-a-Chip published by the Royal Society of Chemistry.

The team developed a protein-protein interaction detection box (LTDMF-PPI-Box) based on a low-temperature digital microfluidic system to achieve automated, rapid, non-destructive, and efficient protein-protein interaction detection.

Protein-protein interaction (PPI) is one of the most fundamental phenomena in living systems, and they play an important role in cells. Studying the interaction between proteins is of great significance for understanding the occurrence and development of diseases, and accurately determining their pathway and mode of action through scientific methods can greatly help biomedical research and drug development such as novel drug target discovery.

At present, common PPI detection methods include tandem affinity purification, fluorescence colocalization, fluorescence bimolecular complementation, fluorescence energy resonance transfer, co-immunoprecipitation and protein chip. However, these methods are often not sufficiently automated to efficiently implement parallel detection; In addition, the current detection technology is insufficient in detection sensitivity and detection speed, which makes it difficult to achieve efficient and accurate PPI measurement.

To solve the above problems, the researchers developed a PPI detection box (LTDMF-PPI-Box) based on a low-temperature digital microfluidic system to achieve automatic, fast, non-destructive and efficient PPI detection.

Low-temperature digital microfluidic (LTDMF) programs the movement of droplets to shorten the detection time of PPI from tens of hours to 1.5 hours. In addition, the integrated thermoelectric cooler can ensure the operating temperature of the protein, increasing the preservation rate of the protein to more than 90%, which is conducive to subsequent operation and application.

Working diagram of PPI detection box based on low-temperature digital microfluidic system

In addition, the team used the interaction between the RILP protein and the Rab26 protein, which is closely related to insulin secretion, as a prototype, further demonstrating the feasibility of the detection box.

According to reports, LTDMF-PPI-Box is composed of two parts: digital microfluidic system and temperature control system. Digital microfluidic system enables accurate sample manipulation and detection; The temperature control system realizes the sample environment control to improve the detection accuracy and feasibility of subsequent applications. Compared with traditional PPI detection methods, LTDMF-PPI-Box proposed by the research team is a highly advantageous protein interaction detection platform, and its automation potential is expected to solve the needs of high-throughput screening of interacting proteins, which is expected to accelerate the establishment of PPI networks in the future. (Source: China Science News, Diao Wenhui)

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