LIFE SCIENCE

Studies have revealed the heat tolerance mechanism of white trifolia


Recently, the Peng Yan/Lizhou team of the College of Pratacultural Science and Technology of Sichuan Agricultural University published a research paper in The Plant Journal, revealing a new mechanism for TrFQR1 to improve the heat tolerance of white clover in cold-season grasses by regulating mitochondrial electron transport, reactive oxygen species homeostasis and lipid remodeling.

Regulation of TrFQR1 gene expression level affects plant heat tolerance Photo courtesy of Sichuan Agricultural University

To investigate the flavin-like oxedentin quinone reductase (FQR) regulating plant adaptability to high-temperature environments, the researchers first identified and cloned a gene encoding FQR, TrFQR1, from white trifolia, and subcellular localization found that the protein encoded by TrFQR1 was located in mitochondria. Yeast overexpressing TrFQR1 had significantly enhanced heat tolerance and improved tolerance to benzoquinone, phenanthraquinone and hydroquinone toxicity. Compared with wild type, transgenic Arabidopsis thaliana and white trifolia overexpressing TrFQR1 showed significantly lower oxidative damage, better photosynthetic ability and growth under high temperature stress, while AtFQR1-RNAi Arabidopsis thaliana showed increased oxidative damage and slower growth under heat stress. The white trifolia that overexpressed TrFQR1 under high temperature stress also maintained a good mitochondrial electron transport chain and a relatively complete mitochondrial ultrastructure.

In addition, the overexpression of TrFQR1 also significantly increased the accumulation of lipids such as phosphatidylglycerol (PG), monogalactosyldiacylglycerol (MGDG), glycolipid sulfate (SQDG) and cardiolipin (CL) in white trifolia under thermal stress, which are involved in the formation of mitochondrial or chloroplast membranes and are associated with improving the stability of the membrane system. White trifolia overexpressing TrFQR1 also exhibited higher lipid saturation levels and a higher ratio of phosphatidylcholine to phosphatidylethanolamine (PC:PE), which may be beneficial for plants to maintain membrane stability and integrity under long-term thermal stress. TrFQR1 can be used as a key candidate gene for screening heat-tolerant genotype materials or breeding heat-tolerant crops through molecular breeding.

TrFQR1 regulates mitochondrial electron transport, reactive oxygen species homeostasis and membrane lipid remodeling to improve plant heat tolerance.    Photo courtesy of Sichuan Agricultural University

Professor Peng Yan and Associate Professor Li Zhou of the College of Pratacultural Science and Technology of Sichuan Agricultural University as co-corresponding authors of the paper, doctoral student Cheng Bizhen as the first author, and master’s students Zhou Min and Tang Tao as co-first authors, the results were supported by the Sichuan Forage Innovation Team of the National Modern Agricultural Industry Technology System and the Sichuan Science and Technology Program. (Source: Zhang Qingdan, China Science News)

Related paper information:https://doi.org/10.1111/tpj.16230



Source link

Related Articles

Leave a Reply

Your email address will not be published. Required fields are marked *

Back to top button