The first kale haploid induction line was successfully created

Cabbage boc03.dmp9 mutant induces the production of maternal haploid Courtesy of the Institute of Vegetable, Chinese Academy of Agricultural Sciences

Recently, the kale and broccoli research group of the Institute of Vegetables and Flowers of the Chinese Academy of Agricultural Sciences successfully obtained the first kale haploid induction system. The results were published in the botanical journal Plant Biotechnology Journal.

Cole vegetables include a variety of important vegetable crops such as cabbage, broccoli, cauliflower, and Brussels sprouts. Currently, cabbage varieties are mostly hybrids, and obtaining purebred parents is a critical step in their breeding. Compared with the traditional method of inbring and backcrossing, the use of double haploid (DH) breeding technology only takes 2 generations to obtain a fully homozygous material, which can greatly shorten the breeding life. The traditional haploid induction technique for kale is in vitro culture of small spores or anthers, which is cumbersome in steps and severely limited by the genotype of the material.

The study cloned a cabbage gene BoC03.DMP9 that was highly expressed in pollen and studied its function using CRISPR/Cas9 gene editing technology.

The results showed that the vegetative growth of the boc03.dmp9 mutant was normal, but the fruiting rate was significantly reduced in the case of inbreeding or as a parent. The induction efficiency of cabbage materials with different backgrounds was studied, and the experiments showed that boc03.dmp9 self-inbred or hybridized as a parent could induce the production of maternal haploids, with an induction rate of 0.41%-2.35%, and no genotype dependence.

This study confirmed the haploid induction function of the DMP9 gene of cabbage, and solved the problem of haploid induction limited by genotype. It has important significance for accelerating the breeding of cabbage, and also provides a reference for the creation of haploid induction lines of other crops.

The paper takes the Institute of Vegetables and Flowers of the Chinese Academy of Agricultural Sciences as the first and corresponding author unit, Zhao Xinyu, a master’s student, and Kevin Yuan, a doctoral student, as the co-first author of the paper, and researcher Lu Honghao and Han Fengqing, an assistant researcher, as the corresponding authors. The research was funded by the Innovation Project of the Chinese Academy of Agricultural Sciences and the National Modern Agricultural Industry Technology System. (Source: China Science Daily, Li Chen)

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