The mechanism of jasmonin regulating tomato anti-root-knot nematodes was revealed

The facility horticulture team of Beijing Agricultural College elucidated that JA finely controlled the content of kaempferol in the lower part of tomato by regulating the flavonol synthesis inhibitor MYB57 and activator MYB108/112 to weigh the development of lateral roots and the improvement of resistance. A few days ago, the relevant research was published in The New Botanist.

A working model of jasmonin regulating tomato resistance to root-knot nematodes. Photo courtesy of the research group

The research team demonstrated that the overexpression of the JA synthetic gene LoxD significantly improved the resistance of tomatoes to nematodes, and the invasion rate of nematode in LoxD gene overexpressed lines was significantly lower than that of wild types. They performed metabolomic analysis of LoxD overexpression and root exudates of wild-type tomatoes and found a significant increase in flavonoids in the root exudates of overexpressed strains.

Through one-on-one experiments on nematode avoidance and motor inhibition, they confirmed that flavonoids (kaempferol) inhibited the motility of nematodes and affected the invasion rate of nematodes. At the same time, they found that LoxD overexpression promoted the significant upregulation of FLS, F3H and CHI gene expression in the kaempferol synthesis pathway, and FLS overexpression strains significantly improved resistance to root-knot nematodes.

They found that the MYB transcription factors MYB57, MYB108 and MYB112 that regulate kaempferol synthesis in tomato roots were all induced by external application of JA and root-knot nematodes. MYB57 interacts with multiple JAZ proteins in the jasmonic acid signaling pathway. MYB57 negatively regulates the synthesis of kaempferol and root-knot nematode resistance, while positively regulates root growth. MYB108 and 112 are the opposite.

The research team said that this shows that kaempferol, as an important secondary metabolite and signaling substance in plants, is strictly regulated. Jasmonic acid signaling simultaneously regulates the level of kaempferol at multiple levels to maintain stress resistance and growth and development balance in the body.

They also discussed the existence of two sides of JA in the interaction between plants and root-knot nematodes. Low concentrations of JA can be used by root-knot nematodes to promote the formation of feeding sites, which is conducive to the parasitism of root-knot nematodes. High concentrations of JA prevent the invasion of root-knot nematodes, inhibit the formation of root knots and the parasitism of nematodes.

It is reported that Professor Wang Shaohui and Associate Professor Zhao Wenchao of Beijing Agricultural College are the corresponding authors of the paper, Liang Jingjing, a doctoral student at China Agricultural University, and Zhao Wenchao and Huang Huang, associate professors of Beijing Agricultural College, are the co-first authors of the paper. (Source: China Science News, Wen Caifei, Zhang Liping)

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